Supposing you want to carry out a unit test from a specific account that is different as the default account, you can basically use the accounts array that is initialized by the truffle development environment and you can explicitly set a specific account as accounts[i] at the function call:
it("test Contract other account", function() {
return Contract.deployed().then(function(instance) {
return Contract.callFunction( <parameters> {from:accounts[i]});
}).then(
...
...